Gateway manual cloning

Invitrogen Gateway recombination cloning uses a one hour reversible recombination reaction, without using restriction enzymes, ligase, subcloning steps, or screening of countless colonies, thereby saving you time, money, and effort. Benefits include: New advancements such as MultiSite Gateway Technology make Gateway cloning the ideal cloning method for protein . The Gateway® Technology is a universal cloning method based on the site-specific recombination properties of bacteriophage lambda Landy, This manual provides an overview of the Gateway File Size: 1MB.  · This manual is available for downloading from our Web site (www.doorway.ru) or by contacting Technical Support (page 20). attP Sequence Variations The attP sites between the pDONR™ vectors will contain. With the Gateway cloning system, a PCR fragment is first cloned into an Entry Vector using standard cloning techniqes (i.e. DNA ligase).

From protein expression to functional analysis, Gateway cloning technology is applicable for a variety of research areas, for truly multidisciplinary scientific studies. Circumvent the roadblocks of traditional restriction enzyme cloning—no need for ligase, subcloning steps, or the hours spent to screen countless colonies. GATEWAY Cloning Technology Instruction Manual, but does not provide rights to synthesize primers or to perform amplification using primers containing recombination sites or portions thereof. Invitrogen Gateway recombination cloning uses a one hour reversible recombination reaction, without using restriction enzymes, ligase, subcloning steps, or screening of countless colonies, thereby saving you time, money, and effort. Benefits include: New advancements such as MultiSite Gateway Technology make Gateway cloning the ideal cloning method for protein expression and functional analysis.

Sept Recombination cloning of single DNA fragments using the Gateway system modified from the Invitrogen MultiSite Gateway Pro user manual. Recombination cloning of single DNA fragments using the Gateway system Schematic modified from the Invitrogen MultiSite Gateway Pro user manual. and their usage, refer to the individual Expression System manual supplied with each kit. Product. Quantity. Catalog no. PCR Cloning System with Gateway®.